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PURPOSE: Vascular endothelial growth factor (VEGF) signal transduction mainly depends on its binding to VEGF receptor 2 (VEGFR-2). VEGF downstream signaling proteins mediate several of its effects in cancer progression, including those on tumor growth, metastasis, and blood vessel formation. The activation of VEGFR-2 signaling is a hallmark of and is considered a therapeutic target for breast cancer. Here, we report a study of the regulation of the VEGFR-2 signaling pathway by a small molecule, isomangiferin. METHODS: A human breast cancer xenograft mouse model was used to investigate the efficacy of isomangiferin in vivo. The inhibitory effect of isomangiferin on breast cancer cells and the underlying mechanism were examined in vitro. RESULTS: Isomangiferin suppressed tumor growth in xenografts. In vitro, isomangiferin treatment inhibited cancer cell proliferation, migration, invasion, and adhesion. The effect of isomangiferin on breast cancer growth was well coordinated with its suppression of angiogenesis. A rat aortic ring assay revealed that isomangiferin significantly inhibited blood vessel formation during VEGF-induced microvessel sprouting. Furthermore, isomangiferin treatment inhibited VEGF-induced proliferation of human umbilical vein endothelial cells and the formation of capillary-like structures. Mechanistically, isomangiferin induced caspase-dependent apoptosis of breast cancer cells. Furthermore, VEGF-induced activation of the VEGFR-2 kinase pathway was down-regulated by isomangiferin. CONCLUSION: Our findings demonstrate that isomangiferin exerts anti-breast cancer effects via the functional inhibition of VEGFR-2. Pharmaceutically targeting VEGFR-2 by isomangiferin could be an effective therapeutic strategy for breast cancer.
Subject(s)
Animals , Humans , Mice , Rats , Angiogenesis Inhibitors , Apoptosis , Blood Vessels , Breast Neoplasms , Cell Proliferation , Heterografts , Human Umbilical Vein Endothelial Cells , In Vitro Techniques , Microvessels , Neoplasm Metastasis , Phosphotransferases , Receptors, Vascular Endothelial Growth Factor , Signal Transduction , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factor Receptor-2ABSTRACT
Objective To investigate the application effect of psychological intervention combined with parecoxib in rehabilitation of patients with bile duct carcinoma after the operation. Methods According to the different intervention models from January 2015 to March 2017 in the first people's hospital of Yongkang city surgical treatment of bile duct carcinoma,40 cases of patients groups: control group with routine nursing plus saline intervention, observation group with psychological nursing + Tenai (parecoxib sodium) mode of intervention; on the psychological state of the two groups before and after intervention to improve patients the cognitive function, to improve the situation, changes in pain scores were recorded, and the related data for comparative analysis. Results The psychological nursing + Tenai (parecoxib sodium) model (observation group) intervention in the treatment of patients with bile duct cancer surgery clinical effect is better than that of routine nursing mode + normal saline (control group) the clinical effect of intervention, the psychological state of patients to improve the situation, improve cognitive function, pain score changes were better than the control group, there was statistical significant differences (P<0.05). Conclusion The surgical treatment of patients with cholangiocarcinoma patients psychological nursing + Tenai (parecoxib sodium) intervention effect significantly, can effectively improve the patients psychological status, cognitive function, and can reduce postoperative pain fully, has an excellent role in promoting the rehabilitation of patients after surgery, worthy of clinical application.
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Objective To investigate the effect of psychological intervention combined with parecoxib on cognitive function in patients after liver resection of hepatocellular carcinoma. Methods From March 2015 to March 2017 in the first people's hospital of Yongkang city for diagnosis and treatment of liver cancer were 40 cases according to different nursing, drug intervention were divided into control group and observation group, the control group were treated with basic nursing + saline intervention, observation group with psychological intervention + Tenai (parecoxib sodium) intervention. The changes of cognitive function (MMSE score), inflammatory factors (TNF-α, IL-6 and S100) levels were recorded before and after intervention in the two groups, and the data obtained were analyzed and summarized. Results The psychological intervention and intervention Tenai (observation group) resection for hepatocellular carcinoma is better than the basic nursing + saline patients (control group) intervention, patients with MMSE score and TNF- α, IL-6, beta S100 levels were better than the control group, the difference was statistically significant (P<0.05). Conclusion Resection for hepatocellular carcinoma patients psychological intervention + Tenai intervention effect, it can reduce the level of inflammatory factors in patients, and has a good effect on the recovery of cognitive function after surgery.
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To compare different nasal cavity nursing methods on mechanically ventilated patients. According to acute physiology and chronic health evaluation [APACHEII], 615 cases of mechanically ventilated patients were divided into group A, group B and group C by stratified random method. Traditional oral nursing plus aspirating secretions from oral cavity and nasal cavity q6h were done in group A. Based on methods in group A, normal saline was used for cleaning nasal cavity in group B. Besides the methods in group A, atomizing nasal cleansing a6h was also used in group C. Incidence rate of Ventilator-Associated Pneumonia [VAP] and APACHE II scores after administrating were compared. The correlation between APACHE II score and outcomes was analyzed by Spearman-rank correlation. In group A, incidence of VAP was 36.76%, group B was 30.24%, group C was 20.38%, and the difference was statistically significant. APACHE II scores in group C were significantly lower compared with group A and B. APACHE II score was negatively correlated with clinical outcomes. For mechanically ventilated patients, nasal nursing can't be ignored and the new atomizing nasal cleaning is an effective method for VAP prevention
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Humans , Female , Male , Pneumonia, Ventilator-Associated , Nasal CavityABSTRACT
OBJECTIVE To investigate the occurrence of injuries by sharp instrument among new medical staff and their preventive consciousness and measures.METHODS Through questionaires,one hundred and fifty four interns who graduated from 2004 to 2006 were surveyed retrospectively.RESULTS Of the new interns,126(81.8%)persons were injured 585 times by sharp instrument,87.8% of whom were injured twice.During the internship,83 persons had passed sharp instrument injury prevention training,and earned priorities in its prevention.In the group of 83 persons trained,59 persons(71.1%) were injured;in another group of 71 persons untrained,67 persons(94.4%) were injured,there was significant difference at the rate of injuries between two groups.Opening ampules,and manipulating needles of syringes or infusion devices were the three major causes due which sharp injuries happened,were accounted for 35%,27% and 22%.CONCLUSIONS New medical staff have high rate of the sharp instrument injuries during internship,the occurrences of injuries are mostly due to the inadequacy in consciousness,the inpreficiency in operating,the high rate of with sharp instrument.Therefore,to reduce the sharp instrument injuries,developing good operating habit;performing operating procedure conscientiously;using medical equipment which can prevent injuries are the effective measures.